日韩夜夜爽I精品主播网红福利资源观看I国产麻豆精品95视频I日日夜avI在线色吧I日韩精品专区

【文獻標題】Environmental concentrations of antibiotics impair zebrafish gut health

【作者】Li Zhou，Samwel Mchele Limbu，Meilin Shen，et.al

【作者單位】華東師范大學（East China Normal University）

【文獻中引用產品】

魚丙二醛（MDA）ELISA試劑盒

【關鍵詞】Antibiotic，Intestinal microbiota，Gut health，Zebrafish

【DOI】doi.org/10.1016/j.envpol.2017.12.073

【影響因子(IF)】5.98

【出版期刊】《Environmental Pollution》

【產品原文引用】

 Biochemical assays

In order to minimize bias, each treatment contained 30 fish for biochemical analysis. The whole gut contents of each fish were weighed and homogenized with 9 vol (v/w) of 0.8% physiological saline. Then, the homogenate was centrifuged at 2500×g at 4℃ for 10 min and the supernatant was collected for biochemical assays according to the manufacturer's instructions. Malondialdehyde (MDA) was measured using an enzyme-linked immune sorbent assay (ELISA) kit (Hengyuan, Shanghai). Superoxide dismutase (SOD), peroxidase (POD), reduced glutathione (GSH), acid phosphatase (ACP), and alkaline phosphatase (AKP) were measured using related commercial assay kits (Nanjing Jiancheng Institute,China). Results were recorded on a microplate reader (Epoch, BioTek, USA).

完整版PDF文獻請咨詢在線客服或者聯XI我司業務員！

更多公司福利請關注“恒遠生物”VX公眾號！